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This is a fluorescence
image of two dendrites from hippocampal neurons growing in cell
culture. One of the cells is expressing actin tagged with green
fluorescent protein (GFP-actin), which giving the green staining
in dendritic spines where actin is highly concentrated (see Kaech
et al, 1997). The culture was fixed and counterstained with an
antibody (red fluorescence) against MAP2, a microtubule-associated
protein which is selectively associated with microtubules in
dendrites and shows the shafts of dendrites. |