You would normally only use this function if you have pre-normalized data, such as data prepared with Affymetrix's Global Scaling?. In that instance, you would want to divide all data by 2500 (or whatever number you chose to normalize by using the Affymetrix software). You will need to do this because the GeneSpring analysis algorithms assume your data is normalized to a median of 1. GeneSpring will use the following formula:
You can use this normalization in concert with Normalize Each Gene to Itself.
Please refer to section The Normalizations: Each Sample to a Hard Number panel will appear. In this panel you tell GeneSpring if you want to normalize your samples to a value you enter. You would normally only use this function if you have pre-normalized data, such as data prepared with Affymetrix's Global Scaling. In that instance, you would want to divide all data by 2500 (or whatever number you chose to normalize by in the Affymetrix software.) You will need to do this because the GeneSpring analysis algorithms assume your data is normalized to a median of 1. or to the Use Constant Values for more details.