Structural biology has become an invaluable tool to gain insight into complex cellular mechanisms and is also widely used in modern structure-based drug discovery. At present structural biology methods include cryo electron microscopy, small angle X-ray scattering, NMR, and X-ray crystallography, with the latter contributing to the vast majority of high-resolution structures in the Protein Data Bank. To cover the increasing demand from FMI research groups for structural biology projects, we have established a Protein Structure Facility (PSF). The facility focuses on protein X-ray crystallography and offers advice as well as hands-on support in all aspects from target analysis to the final 3D-structure. We run a complete modern wet lab for protein production and use state-of-the-art technology for high-throughput protein crystallization, data collection in-house and at the synchrotron, as well as structure solution. We are committed to providing cutting-edge methods at every stage of the structure determination pipeline to make it as efficient as possible.