Cell sorting (FACS)

Flow cytometry provides an incredibly flexible method for the characterization of cells in suspension. Fluorescence activated cell sorting (FACS) allows selection of cells on the basis of e.g. surface, cytoplasmic and nuclear antigens, GFP expression, DNA content, cell cycle phase, apoptosis characters, etc, etc. The FMI FACS service uses two instruments: the FACSCalibur, a two-laser machine allowing two light-scatter parameters and four fluorescence channels to be measured, and the MoFlo high-speed cell sorter equipped with 3 lasers.