C. elegans facility
Assistance with all aspects of work on the nematode model organism as well as hydra, incl. focus on CRISPR/cas tools
Since the 1960s, the nematode Caenorhabditis elegans has been used as model organisms for the investigation of a variety of biological processes. As a transparent, multi-cellular, eukaryotic organism, C. elegans not only facilitates the study of cellular differentiation and other developmental processes, it is also an excellent genetic model system. At the Friedrich Miescher Institute, groups investigating epigenetics and signaling work with C. elegans.
The C. elegans facility assists with all aspects of work on this nematode model organism. The facility
- assists groups working primarily on Caenorhabditis elegans with genetic screens, nematode techniques, and reagents.
- develops and implements new methods.
- acts as an entry point for all FMI investigators wishing to use C. elegans in any aspect of their research.
Iskra Katic: Platform head, worm platform
Padeken J, Zeller P, Towbin B, Katic I, Kalck V, Methot SP, Gasser SM (2019) Synergistic lethality between BRCA1 and H3K9me2 loss reflects satellite derepression.Genes Dev. 2019 Apr 1;33(7-8):436-451
Delaney CE, Methot SP, Guidi M, Katic I, Gasser SM, Padeken J (2019) Heterochromatic foci and transcriptional repression by an unstructured MET-2/SETDB1 co-factor LIN-65.J Cell Biol. 2019 Mar 4;218(3):820-838
Aeschimann F, Kumari P, Bartake H, Gaidatzis D, Xu L, Ciosk R, Großhans H (2017) LIN41 Post-transcriptionally Silences mRNAs by Two Distinct and Position-Dependent Mechanisms.Mol Cell. 2017 Feb 2;65(3):476-489.e4
Ewald CY, Hourihan JM, Bland MS, Obieglo C, Katic I, Moronetti Mazzeo LE, Alcedo J, Blackwell TK, Hynes NE (2017) NADPH oxidase-mediated redox signaling promotes oxidative stress resistance and longevity through <i>memo-1</i> in <i>C. elegans</i>.Elife. 2017 Jan 13;6
Richter H, Katic I, Gut H, Großhans H (2016) Structural basis and function of XRN2 binding by XTB domains.Nat Struct Mol Biol. 2016 Feb;23(2):164-71
Katic I, Xu L, Ciosk R (2015) CRISPR/Cas9 Genome Editing in Caenorhabditis elegans: Evaluation of Templates for Homology-Mediated Repair and Knock-Ins by Homology-Independent DNA Repair.G3 (Bethesda). 2015 Jun 3;5(8):1649-56
Arnold A, Rahman MM, Lee MC, Muehlhaeusser S, Katic I, Gaidatzis D, Hess D, Scheckel C, Wright JE, Stetak A, Boag PR, Ciosk R (2014) Functional characterization of C. elegans Y-box-binding proteins reveals tissue-specific functions and a critical role in the formation of polysomes.Nucleic Acids Res. 2014 Dec 1;42(21):13353-69
Katic I, Großhans H (2013) Targeted heritable mutation and gene conversion by Cas9-CRISPR in Caenorhabditis elegans.Genetics. 2013 Nov;195(3):1173-6