Cell sorting (FACS)

Cell sorting (FACS)

A sophisticated method for the characterization and isolation of subpopulations of cells identified by multiparameter analysis

The facility provides a wide range of flow cytometric techniques that allow various experimental approaches to research problems. We operate one analytical instrument and three preparative cell sorters from Becton Dickinson and SONY, and offer a service that is comprehensive, flexible and user-friendly, using high-end, state-of-the-art methodologies. We also provide training, assistance and technical support, including advice on the experimental design, sample handling, data collection, analysis and interpretation.

Hubertus Kohler: Platform head, cell sorting

Gill ME, Kohler H, Peters AHFM (2024) Isolation of Mouse Germ Cells by FACS Using Hoechst 33342 and SYTO16 Double Staining.

Methods Mol Biol. 2024;2770:53-62

Hamelin B, Obradovic MMS, Sethi A, Kloc M, Münst S, Beisel C, Eschbach K, Kohler H, Soysal S, Vetter M, Weber WP, Stadler MB, Bentires-Alj M (2023) Single-cell Analysis Reveals Inter- and Intratumour Heterogeneity in Metastatic Breast Cancer.

J Mammary Gland Biol Neoplasia. 2023 Dec 8;28(1):26

Kessler S, Minoux M, Joshi O, Ben Zouari Y, Ducret S, Ross F, Vilain N, Salvi A, Wolff J, Kohler H, Stadler MB, Rijli FM (2023) A multiple super-enhancer region establishes inter-TAD interactions and controls Hoxa function in cranial neural crest.

Nat Commun. 2023 Jun 5;14(1):3242

Suppinger S, Zinner M, Aizarani N, Lukonin I, Ortiz R, Azzi C, Stadler MB, Vianello S, Palla G, Kohler H, Mayran A, Lutolf MP, Liberali P (2023) Multimodal characterization of murine gastruloid development.

Cell Stem Cell. 2023 Jun 1;30(6):867-884.e11

Gill ME, Kohler H, Peters AHFM (2022) Dual DNA staining enables isolation of multiple sub-types of post-replicative mouse male germ cells.

Cytometry A. 2022 Jun;101(6):529-536

Zuin J, Roth G, Zhan Y, Cramard J, Redolfi J, Piskadlo E, Mach P, Kryzhanovska M, Tihanyi G, Kohler H, Eder M, Leemans C, van Steensel B, Meister P, Smallwood S, Giorgetti L (2022) Nonlinear control of transcription through enhancer-promoter interactions.

Nature. 2022 Apr;604(7906):571-577

Methot SP, Padeken J, Brancati G, Zeller P, Delaney CE, Gaidatzis D, Kohler H, van Oudenaarden A, Großhans H, Gasser SM (2021) H3K9me selectively blocks transcription factor activity and ensures differentiated tissue integrity.

Nat Cell Biol. 2021 Nov;23(11):1163-1175

Song S, Cao C, Choukrallah MA, Tang F, Christofori G, Kohler H, Wu F, Fodor BD, Frederiksen M, Willis SN, Jackson JT, Nutt SL, Dirnhofer S, Stadler MB, Matthias P (2021) OBF1 and Oct factors control the germinal center transcriptional program.

Blood. 2021 May 27;137(21):2920-2934

Kitazawa T, Machlab D, Joshi O, Maiorano N, Kohler H, Ducret S, Kessler S, Gezelius H, Soneson C, Papasaikas P, López-Bendito G, Stadler MB, Rijli FM (2021) A unique bipartite Polycomb signature regulates stimulus-response transcription during development.

Nat Genet. 2021 Mar;53(3):379-391

Maheshwari U, Kraus D, Vilain N, Holwerda SJB, Cankovic V, Maiorano NA, Kohler H, Satoh D, Sigrist M, Arber S, Kratochwil CF, Di Meglio T, Ducret S, Rijli FM (2020) Postmitotic Hoxa5 Expression Specifies Pontine Neuron Positional Identity and Input Connectivity of Cortical Afferent Subsets

Cell Rep. 2020 Jun 16;31(11):107767

Obradovic MMS, Hamelin B, Manevski N, Couto JP, Sethi A, Coissieux MM, Münst S, Okamoto R, Kohler H, Schmidt A, Bentires-Alj M (2019) Glucocorticoids promote breast cancer metastasis

Nature. 2019 Mar;567(7749):540-544

Moncayo G, Grzmil M, Smirnova T, Zmarz P, Huber RM, Hynx D, Kohler H, Wang Y, Hotz HR, Hynes NE, Keller G, Frank S, Merlo A, Hemmings BA (2018) SYK inhibition blocks proliferation and migration of glioma cells and modifies the tumor microenvironment.

Neuro Oncol. 2018 Apr 9;20(5):621-631

Daum JM, Keles Ö, Holwerda SJ, Kohler H, Rijli FM, Stadler M, Roska B (2017) The formation of the light-sensing compartment of cone photoreceptors coincides with a transcriptional switch

Elife. 2017 Nov 6;6

Minoux M, Holwerda S, Vitobello A, Kitazawa T, Kohler H, Stadler MB, Rijli FM (2017) Gene bivalency at Polycomb domains regulates cranial neural crest positional identity

Science. 2017 Mar 31;355(6332)

Koren S, Reavie L, Couto JP, De Silva D, Stadler MB, Roloff T, Britschgi A, Eichlisberger T, Kohler H, Aina O, Cardiff RD, Bentires-Alj M (2015) PIK3CA(H1047R) induces multipotency and multi-lineage mammary tumours.

Nature. 2015 Sep 3;525(7567):114-8

Cronin T, Vandenberghe LH, Hantz P, Juttner J, Reimann A, Kacsó AE, Huckfeldt RM, Busskamp V, Kohler H, Lagali PS, Roska B, Bennett J (2014) Efficient transduction and optogenetic stimulation of retinal bipolar cells by a synthetic adeno-associated virus capsid and promoter.

EMBO Mol Med. 2014 Sep;6(9):1175-90

Du Roure C, Versavel A, Doll T, Cao C, Pillonel V, Matthias G, Kaller M, Spetz JF, Kopp P, Kohler H, Müller M, Matthias P (2014) Hematopoietic overexpression of FOG1 does not affect B-cells but reduces the number of circulating eosinophils.

PLoS One. 2014;9(4):e92836

Yokobayashi S, Liang CY, Kohler H, Nestorov P, Liu Z, Vidal M, van Lohuizen M, Roloff TC, Peters AH (2013) PRC1 coordinates timing of sexual differentiation of female primordial germ cells

Nature. 2013 Mar 14;495(7440):236-40

Milani ES, Brinkhaus H, Dueggeli R, Klebba I, Mueller U, Stadler M, Kohler H, Smalley MJ, Bentires-Alj M (2013) Protein tyrosine phosphatase 1B restrains mammary alveologenesis and secretory differentiation

Development. 2013 Jan 1;140(1):117-25

Lai JC, Ponti S, Pan D, Kohler H, Skoda RC, Matthias P, Nagamine Y (2012) The DEAH-box helicase RHAU is an essential gene and critical for mouse hematopoiesis

Blood. 2012 May 3;119(18):4291-300

Siegert S, Cabuy E, Scherf BG, Kohler H, Panda S, Le YZ, Fehling HJ, Gaidatzis D, Stadler MB, Roska B (2012) Transcriptional code and disease map for adult retinal cell types

Nat Neurosci. 2012 Jan 22;15(3):487-95, S1-2

Schwaiger M, Kohler H, Oakeley EJ, Stadler MB, Schübeler D (2010) Heterochromatin protein 1 (HP1) modulates replication timing of the Drosophila genome.

Genome Res. 2010 Jun;20(6):771-80

Yamaguchi T, Cubizolles F, Zhang Y, Reichert N, Kohler H, Seiser C, Matthias P (2010) Histone deacetylases 1 and 2 act in concert to promote the G1-to-S progression.

Genes Dev. 2010 Mar 1;24(5):455-69

Schwaiger M, Stadler MB, Bell O, Kohler H, Oakeley EJ, Schübeler D (2009) Chromatin state marks cell-type- and gender-specific replication of the Drosophila genome.

Genes Dev. 2009 Mar 1;23(5):589-601

Bordon A, Bosco N, Du Roure C, Bartholdy B, Kohler H, Matthias G, Rolink AG, Matthias P (2008) Enforced expression of the transcriptional coactivator OBF1 impairs B cell differentiation at the earliest stage of development.

PLoS One. 2008;3(12):e4007

Roelandse M, Welman A, Wagner U, Hagmann J, Matus A (2003) Focal motility determines the geometry of dendritic spines.

Neuroscience. 2003;121(1):39-49

Welman A, Burger MM, Hagmann J (2000) Structure and function of the C-terminal hypervariable region of K-Ras4B in plasma membrane targetting and transformation.

Oncogene. 2000 Sep 21;19(40):4582-91

Hagmann J, Burger MM, Dagan D (1999) Regulation of plasma membrane blebbing by the cytoskeleton.

J Cell Biochem. 1999 Jun 15;73(4):488-99


Platform/Service Head

In current position since 2002
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Biography of Hubertus Kohler

Positions held

Head FACS Facility, Friedrich Miescher Institute for Biomedical research, Basel Switzerland
Head FACS Facility, Basel Institute for Immunology and Basilea Pharmaceutica AG, Switzerland
Cytometry Specialist FACS Facility, Max-Planck-Institute of Immunobiology and Epigenetics, Freiburg i.Br. Germany