The Structural Biology Platform (SBP) supports FMI scientists with cutting-edge structural biology techniques including cryo-electron microscopy (cryo-EM) and X-ray crystallography. In addition, we assist FMI users with modern computational methods for the analysis of protein structures, model building and final structure validation.

Since 2016, we have been working together with the EM team at Novartis to first establish and then further develop and expand a joint EM facility. The expertise in the EM facility is complemented by the FAIM platform at FMI, bringing scanning electron microscopy competence.

Single-particle cryo-electron microscopy
With the introduction of a new generation of direct electron detectors for transmission electron microscopes, followed by a development of new software for image analysis and increased computational power, we are now in a position to image ice-embedded molecular machines at near-atomic resolution. We use single-particle cryo-electron microscopy (cryo-EM) to reveal structural information and mode of action of pivotal protein complexes. Cryo-EM does not require crystallization and can be applied to protein complexes with a broad range of molecular weights. Dedicated to support FMI research groups, we provide service and training from sample preparation to image analysis.

X-Ray crystallography
For several decades, X-ray crystallography has been the standard technique to solve high- resolution structures. X-ray crystallography is an important tool to obtain high-resolution structures of small to medium sized proteins, nucleic acids and small complexes. It complements the accurate analysis of large macromolecular complexes by cryo-EM, which often relies on crystal structures of individual subcomponents. We provide equipment for convenient high-throughput crystal screening and regular synchrotron access in collaboration with Novartis. Our expertise and hands-on support help FMI research groups to carry out successful crystallography projects.

Computational structural biology
We apply cutting-edge computational methods for structure prediction, docking, and molecular dynamics simulations. These methods are often combined with low-resolution experimental information and help to guide further experiments. We also support FMI research groups interested in using these methods independently.

Cloning and protein production
Routine protein purification and cloning based on established protocols are offered as a support to research groups at the FMI. The platform is equipped to maintain E. coli, insect and mammalian cells culture.

Contact
Simone Cavadini: Platform head, structural biology