Structural Biology

Structural Biology

Cryo-electron microscopy, X-ray crystallography and computational methods for high resolution structural characterization of protein

The Structural Biology Platform (SBP) supports FMI scientists with cutting-edge structural biology techniques including cryo-electron microscopy (cryo-EM) and X-ray crystallography. In addition, we assist FMI users with modern computational methods for the analysis of protein structures, model building and final structure validation.

Since 2016, we have been working together with the EM team at Novartis to first establish and then further develop and expand a joint EM facility. The expertise in the EM facility is complemented by the FAIM platform at FMI, bringing scanning electron microscopy competence.

Single-particle cryo-electron microscopy
With the introduction of a new generation of direct electron detectors for transmission electron microscopes, followed by a development of new software for image analysis and increased computational power, we are now in a position to image ice-embedded molecular machines at near-atomic resolution. We use single-particle cryo-electron microscopy (cryo-EM) to reveal structural information and mode of action of pivotal protein complexes. Cryo-EM does not require crystallization and can be applied to protein complexes with a broad range of molecular weights. Dedicated to support FMI research groups, we provide service and training from sample preparation to image analysis.

X-Ray crystallography
For several decades, X-ray crystallography has been the standard technique to solve high- resolution structures. X-ray crystallography is an important tool to obtain high-resolution structures of small to medium sized proteins, nucleic acids and small complexes. It complements the accurate analysis of large macromolecular complexes by cryo-EM, which often relies on crystal structures of individual subcomponents. We provide equipment for convenient high-throughput crystal screening and regular synchrotron access in collaboration with Novartis. Our expertise and hands-on support help FMI research groups to carry out successful crystallography projects.

Computational structural biology
We apply cutting-edge computational methods for structure prediction, docking, and molecular dynamics simulations. These methods are often combined with low-resolution experimental information and help to guide further experiments. We also support FMI research groups interested in using these methods independently.

Cloning and protein production
Routine protein purification and cloning based on established protocols are offered as a support to research groups at the FMI. The platform is equipped to maintain E. coli, insect and mammalian cells culture.

Contact
Simone Cavadini: Platform head, structural biology

Pathare GR, Decout A, Glück S, Cavadini S, Makasheva K, Hovius R, Kempf G, Weiss J, Kozicka Z, Guey B, Melenec P, Fierz B, Thomä NH, Ablasser A (2020) Structural mechanism of cGAS inhibition by the nucleosome.

Nature. 2020 Sep 10

Michael AK, Grand RS, Isbel L, Cavadini S, Kozicka Z, Kempf G, Bunker RD, Schenk AD, Graff-Meyer A, Pathare GR, Weiss J, Matsumoto S, Burger L, Schübeler D, Thomä NH (2020) Mechanisms of OCT4-SOX2 motif readout on nucleosomes.

Science. 2020 Jun 26;368(6498):1460-1465

Schenk AD, Cavadini S, Thomä NH, Genoud C (2020) Live Analysis and Reconstruction of Single-Particle Cryo-Electron Microscopy Data with CryoFLARE.

J Chem Inf Model. 2020 May 26;60(5):2561-2569

Bhaskar V, Graff-Meyer A, Schenk AD, Cavadini S, von Loeffelholz O, Natchiar SK, Artus-Revel CG, Hotz HR, Bretones G, Klaholz BP, Chao JA (2020) Dynamics of uS19 C-Terminal Tail during the Translation Elongation Cycle in Human Ribosomes.

Cell Rep. 2020 Apr 7;31(1):107473

Rabl J, Bunker RD, Schenk AD, Cavadini S, Gill ME, Abdulrahman W, Andrés-Pons A, Luijsterburg MS, Ibrahim AFM, Branigan E, Aguirre JD, Marceau AH, Guérillon C, Bouwmeester T, Hassiepen U, Peters AHFM, Renatus M, Gelman L, Rubin SM, Mailand N, van Attikum H, Hay RT, Thomä NH (2019) Structural Basis of BRCC36 Function in DNA Repair and Immune Regulation.

Mol Cell. 2019 Aug 8;75(3):483-497.e9

Matsumoto S, Cavadini S, Bunker RD, Grand RS, Potenza A, Rabl J, Yamamoto J, Schenk AD, Schübeler D, Iwai S, Sugasawa K, Kurumizaka H, Thomä NH (2019) DNA damage detection in nucleosomes involves DNA register shifting.

Nature. 2019 Jul;571(7763):79-84

Members

Platform/Service Head

Simone Cavadini (Platform head, structural biology)

Technical/Research associates

Georg Kempf (Research associate, platform)
Sandra Muehlhaeusser (Lab manager)
Andreas Daniel Schenk (Project leader)

Alumni

Biography of Simone Cavadini

Education

2005-2009
PhD in Physical Chemistry - Ecole Polytechnique Fédérale de Lausanne (EPFL), Switzerland
2000-2005
MSc in Molecular & Biological Chemistry - EPFL, Switzerland

Positions held

2020-
Head of Structural Biology Platform, Friedrich Miescher Institute for Biomedical Research
2016-2020
Project Leader, Friedrich Miescher Institute for Biomedical Research
2014-2016
Project Leader in the Dr. N. H. Thomä group, Friedrich Miescher Institute for Biomedical Research
2010-2014
Postdoctoral fellow, N. H. Thomä group, Friedrich Miescher Institute for Biomedical Research
2010
Mass spectrometry scientist, Institut Biochimique SA, Manno, Switzerland
2005-2009
PhD student, Laboratory of Biomolecular Magnetic Resonance at EPFL, Lausanne, Switzerland

Software and Tools

Test - Not in use for now