Yoshikuni Nagamine
Yoshikuni Nagamine
Signal transduction and the plasminogen activator system
The plasminogen activator (PA) system plays an important role in processes involving extracellular proteolysis, such as angiogenesis, wound healing, gametogenesis, inflammation and tumor metastasis.
Recent results show a new form of biological regulation by the PA system not involving proteolytic activity in which urokinase-type PA (uPA)-occupied uPA receptor acquires a high affinity for vitronectin, promoting cell adhesion or migration on vitronectin-containing extracellular matrix. Studying regulation of uPA and PA inhibitor-1 by growth factors, we found serine/threonine phosphorylation of the adaptor/docking protein ShcA. Tyrosine phosphorylation of ShcA and its role in signaling have been well documented, but its serine/threonine phosphorylation has been little studied. Our work has revealed a role for serine phosphorylation of ShcA in insulin induced signaling.
Faisal A, Kleiner S, Nagamine Y (2004) Non-redundant role of Shc in Erk activation by cytoskeletal reorganization
J Biol Chem 279:3202-3211Tran H, Schilling M, Wirbelauer C, Hess D, Nagamine Y (2004) Facilitation of mRNA deadenylation and decay by the exosome-bound, DExH protein RHAU
Mol Cell 13:101-111Venugopal J, Hanashiro K, Yang ZZ, Nagamine Y (2004) Identification and modulation of a caveolae-dependent signal pathway that regulates plasminogen activator inhibitor-1 in insulin-resistant adipocytes
Proc Nat Acad Sci USA 101:17120-17125Lopez-Alemany R, Redondo J M, Nagamine Y, Munoz-Canoves P (2003) Plasminogen activator inhibitor type-1 inhibits insulin signaling by competing with avbeta3 integrin for vitronectin binding
Eur J Biochem 270:814-821Tran H, Maurer F, Nagamine Y (2003) Stabilization of urokinase and urokinase receptor mRNAs by HuR is linked to its cytoplasmic accumulation induced by activated mitogen-activated protein kinase-activated protein kinase 2
Mol Cell.Biol 23:7177-7188Faisal F, El-Shemerly M, Hess D, Nagamine Y (2002) Serine/threonine phosphorylation of ShcA: regulation of PTP-PEST binding and involvement in insulin signaling
J Biol Chem 277:30144-30152Kisielow M, Kleiner S, Nagasawa M, Faisal A, Nagamine Y (2002) Isoform-specific knock-down and expression of adaptor protein ShcA using small interfering RNA
Biochem J 363:1-5