Jeffrey A. Chao
Group News
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Dec 14, 2020 Thinking afresh about how cells respond to stress |
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Dec 16, 2019 A new druggable step in the gene expression pathway |
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Jan 28, 2019 How do mRNAs deal with stress? |
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Nov 2, 2017 Meet the Author: Ivana Horvathova and Franka Voigt |
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Oct 31, 2017 An RNA TREAT for Halloween |
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Jeffrey A. Chao
Regulation of gene expression
Gene expression requires an orchestrated series of events that is regulated temporally and spatially within the cell. After transcription, an mRNA must be spliced, processed, exported, transported and translated before it is eventually degraded. At each step, the factors that control the fate of the mRNA in either the nucleus or cytoplasm must be appropriately assembled, disassembled or remodeled. Not only is the composition of these macromolecular complexes dynamic, but there are also over 2000 proteins in the human genome involved in RNA metabolism, which makes them extremely heterogeneous as well. In order to better understand the molecular underpinnings that regulate these processes and how these complexes function within their cellular context, our research combines biochemical and structural (X-ray crystallography) techniques with single-molecule imaging of mRNAs in living cells.
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Jeffrey A. Chao
This is a list of selected publications from this group. For a full list of publications, please visit our Publications page and search by group name.
Voigt, F.*, Gerbracht, J.V.*, Böhm, V., Horvathova, I., Eglinger, J., Chao, J.A.#, Gehring, N.H.# (2019) Detection and quantification of RNA decay intermediates using XRN1-resistant reporter transcripts
Nat Protocols. 14(5):1603-1633# Co-corresponding authors. * joint first author
Wilbertz, J.H., Voigt, F. Horvathova, I., Roth, G. Zhan, Y., Chao, J.A. (2019) Single-molecule imaging of mRNA localization and regulation during the integrated stress response
Mol Cell. 73(5): 946-985Jia, M., Gut, H., Chao, J.A. (2018) Structural basis of IMP3 RRM12 recognition of RNA
RNA. 24(12):1659-1666Voigt, F.*, Zhang H.*, Cui, X.A., Triebold, D., Liu, A.X., Eglinger, J., Lee, E.S., Chao, J.A.*#, Palazzo, A.F.* (2017) Single-molecule quantification of translation-dependent association of mRNAs with the endoplasmic reticulum
Cell Reports. 21(13):3740-3753# Lead contact. * joint first author
Horvathova I*, Voigt F*, Kotrys AV, Zhan Y, Artus-Revel CG, Eglinger J, Stadler MB, Giorgetti L, Chao JA (2017) The dynamics of mRNA turnover revealed by single-molecule imaging in single cells
Mol Cell 68:615-625* joint first author
Halstead JM, Lionnet T, Wilbertz JH, Wippich F, Ephrussi A, Singer RH, Chao JA (2015) An RNA biosensor for imaging the first round of translation from single cells to living animals
Science 347:1367-1370Full list of publications
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